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Biomechanical characterzation of endothelial glycocalyx layer

Schematics of the particle probe RICM technique  to measure micro-mechanical properties of glycocalyx (not drawn to scale) (V. Hlady, unpublished).

Fig 1. The basis of RICM measurements: spherical probe samples the underlying cell structure (upper left; not drawn to scale); the RICM image of the actual glass sphere (r = 5 µm) showing the interference fringes spaced at 1/4 of the wavelength of the light (middle left panel); the intensity profile across the RICM image (lower left panel).  Intensity variation in the linear region between the first bright and dark region in the RICM image reveals the fluctuation in the probe vertical position in time, This intensity variation is converted into vertical position variation h(t) from which the potential energy profile, V(h), is calculated (V. Hlady, unpublished).

The potential energy profile, V(h), calculated from RICM intensity fluctuations, are used to calculate the effective spring constant of the bead-cell contact region before and after digesting endothelial cell glycocalyx with specific enzymes (Koskimaki, Dull, Hlady, unpublished).