Analysis of protein adsorption kinetics

Quantitative Analysis of Protein Adsorption Kinetics

(written in collaboration with C.-H. Ho, P.Dryden and D. W. Britt , Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA).

Click here for .pdf copy of the coursenotes (1.7 MB) on mass transport effect on protein adsorption)

To download the Maple codes that will compute protein adsorption across a boundary layer using Corsel et al. model described below, click here: http://afm1.pharm.utah.edu/~vhlady/Maple_code_kinetics

Introduction, Protein adsorption isotherms
Protein adsorption kinetics, Protein-surface interactions, Methods used to study protein adsorption
Quantitative analysis of the protein adsorption kinetics
- Computation of the flux of protein molecules to the adsorbent surface
- Quantification of protein adsorption kinetics based on the transport-limited adsorption from flowing solution
- Quantification of protein adsorption kinetics based on one point measurement using radiolabeled protein
Example: Modeling the kinetics of lipoprotein adsorption
1. Step 1. Finding the desorption rate constant
2. Step 2. Estimates for other fitting parameters
3. Step 3. Interpretation of the parameters obtained by fitting
Adsorption cooperativity, Coda,
References